ProductsBack/Robo Polyclonal Antibody

Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; \nAntigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum 5%) at 37℃ for 20 min; \nIncubation: Anti-Robo Polyclonal Antibody, Unconjugated (bs-5794R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.

$Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; \nAntigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum 5%) at 37℃ for 20 min; \nIncubation: Anti-Robo Polyclonal Antibody, Unconjugated (bs-5794R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.$

Robo Polyclonal Antibody

BS-5794R

ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Product group Antibodies

TargetROBO1

Overview

Supplier
Bioss Antibodies
Product Name
Robo1/2 Polyclonal Antibody
Delivery Days Customer
16
Applications
ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Applications Supplier
WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
Certification
Research Use Only
Clonality
Polyclonal
Concentration
1 ug/ul
Conjugate
Unconjugated
Gene ID6091
Target name
ROBO1
Target description
roundabout guidance receptor 1
Target synonyms
deleted in U twenty twenty; DUTT1; roundabout homolog 1; roundabout, axon guidance receptor, homolog 1; SAX3
Host
Rabbit
Isotype
IgG
Storage Instruction
-20°C
UNSPSC
12352203