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Immunohistochemical staining of human eye, retina shows strong cytoplasmic positivity.
Immunohistochemical staining of human eye, retina shows strong cytoplasmic positivity.
Immunohistochemical staining of human eye, retina shows strong cytoplasmic positivity.

Anti-RPGR Antibody

HPA001593
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPGR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPGR
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6103
  • Target name
    RPGR
  • Target description
    retinitis pigmentosa GTPase regulator
  • Target synonyms
    COD1; CORDX1; CRD; orf15; PCDX; retinitis pigmentosa 15; retinitis pigmentosa 3 GTPase regulator; RP15; RP3; X-linked retinitis pigmentosa GTPase regulator; XLRP3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92834
  • Protein Name
    X-linked retinitis pigmentosa GTPase regulator
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPGR using anti-RPGR antibody (A01522). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGR antigen affinity purified polyclonal antibody (Catalog # A01522) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGR at approximately 113KD. The expected band size for RPGR is at 113KD.
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