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Western blot All lanes: RPGR antibody at 6microg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 114, 91, 71, 84, 53, 128 kDa Observed band size: 114 kDa
Western blot All lanes: RPGR antibody at 6microg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 114, 91, 71, 84, 53, 128 kDa Observed band size: 114 kDa
Western blot All lanes: RPGR antibody at 6microg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 114, 91, 71, 84, 53, 128 kDa Observed band size: 114 kDa

RPGR Antibody

CSB-PA838814LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPGR
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPGR Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6103
  • Target name
    RPGR
  • Target description
    retinitis pigmentosa GTPase regulator
  • Target synonyms
    COD1; CORDX1; CRD; orf15; PCDX; retinitis pigmentosa 15; retinitis pigmentosa 3 GTPase regulator; RP15; RP3; X-linked retinitis pigmentosa GTPase regulator; XLRP3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92834
  • Protein Name
    X-linked retinitis pigmentosa GTPase regulator
  • Scientific Description
    Could be a guanine-nucleotide releasing factor. Plays a role in ciliogenesis. Probably regulates cilia formation by regulating actin stress filaments and cell contractility. Plays an important role in photoreceptor integrity. May play a critical role in spermatogenesis and in intraflagellar transport processes (By similarity). May be involved in microtubule organization and regulation of transport in primary cilia.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPGR using anti-RPGR antibody (A01522). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGR antigen affinity purified polyclonal antibody (Catalog # A01522) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGR at approximately 113KD. The expected band size for RPGR is at 113KD.
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