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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in seminiferous ducts cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in seminiferous ducts cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in seminiferous ducts cells.

Anti-RTN4 Antibody

HPA023977
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRTN4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RTN4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57142
  • Target name
    RTN4
  • Target description
    reticulon 4
  • Target synonyms
    ASY, NI220/250, NOGO, NOGOA, NOGOB, NSP, NSP-CL, Nbla00271, Nbla10545, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, reticulon-4, Human NogoA, My043 protein, foocen, neurite growth inhibitor 220, neurite outgrowth inhibitor, neuroendocrine-specific protein C homolog, reticulon 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQC3
  • Protein Name
    Reticulon-4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Nogo A/RTN4 using anti-Nogo A/RTN4 antibody (A01844-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nogo A/RTN4 antigen affinity purified polyclonal antibody (Catalog # A01844-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nogo A/RTN4 at approximately 180KD. The expected band size for Nogo A/RTN4 is at 180KD.
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