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ProductsBack/RTN4 / Nogo Antibody

RTN4 / Nogo Antibody

LS-C402720
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRTN4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RTN4 / Nogo Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57142
  • Target name
    RTN4
  • Target description
    reticulon 4
  • Target synonyms
    ASY, NI220/250, NOGO, NOGOA, NOGOB, NSP, NSP-CL, Nbla00271, Nbla10545, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, reticulon-4, Human NogoA, My043 protein, foocen, neurite growth inhibitor 220, neurite outgrowth inhibitor, neuroendocrine-specific protein C homolog, reticulon 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nogo A/RTN4 using anti-Nogo A/RTN4 antibody (A01844-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nogo A/RTN4 antigen affinity purified polyclonal antibody (Catalog # A01844-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nogo A/RTN4 at approximately 180KD. The expected band size for Nogo A/RTN4 is at 180KD.
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