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Immunohistochemistry analysis in human lymph node and kidney tissues using HPA059006 antibody. Corresponding RUNX3 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human lymph node and kidney tissues using HPA059006 antibody. Corresponding RUNX3 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human lymph node and kidney tissues using HPA059006 antibody. Corresponding RUNX3 RNA-seq data are presented for the same tissues.

Anti-RUNX3 Antibody

HPA059006
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRUNX3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RUNX3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID864
  • Target name
    RUNX3
  • Target description
    RUNX family transcription factor 3
  • Target synonyms
    AML2, CBFA3, PEBP2aC, runt-related transcription factor 3, CBF-alpha-3, PEA2 alpha C, PEBP2 alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, acute myeloid leukemia 2 protein, acute myeloid leukemia gene 2, core-binding factor subunit alpha-3, core-binding factor, runt domain, alpha subunit 3, oncogene AML-2, polyomavirus enhancer-binding protein 2 alpha C subunit, runt related transcription factor 3, transcription factor AML2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13761
  • Protein Name
    Runt-related transcription factor 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RUNX3 using anti-RUNX3 antibody (PB9432). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX3 antigen affinity purified polyclonal antibody (Catalog # PB9432) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX3 at approximately 43-48 kDa. The expected band size for RUNX3 is at 44 kDa.
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ELISA analysis of antigen using GTX60639 RUNX3 antibody [2B3]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
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