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Western Blot analysis of HuvEc cells using RUNX3 Polyclonal Antibody
Western Blot analysis of HuvEc cells using RUNX3 Polyclonal Antibody
Western Blot analysis of HuvEc cells using RUNX3 Polyclonal Antibody

RUNX3 Antibody

CSB-PA004039
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetRUNX3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RUNX3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID864
  • Target name
    RUNX3
  • Target description
    RUNX family transcription factor 3
  • Target synonyms
    AML2, CBFA3, PEBP2aC, runt-related transcription factor 3, CBF-alpha-3, PEA2 alpha C, PEBP2 alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, acute myeloid leukemia 2 protein, acute myeloid leukemia gene 2, core-binding factor subunit alpha-3, core-binding factor, runt domain, alpha subunit 3, oncogene AML-2, polyomavirus enhancer-binding protein 2 alpha C subunit, runt related transcription factor 3, transcription factor AML2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13761
  • Protein Name
    Runt-related transcription factor 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RUNX3 using anti-RUNX3 antibody (PB9432). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX3 antigen affinity purified polyclonal antibody (Catalog # PB9432) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX3 at approximately 43-48 kDa. The expected band size for RUNX3 is at 44 kDa.
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ELISA analysis of antigen using GTX60639 RUNX3 antibody [2B3]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
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