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Immunohistochemistry analysis in human cerebral cortex and kidney tissues using AMAb91038 antibody. Corresponding S100B RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and kidney tissues using AMAb91038 antibody. Corresponding S100B RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and kidney tissues using AMAb91038 antibody. Corresponding S100B RNA-seq data are presented for the same tissues.

Anti-S100B Antibody

AMAB91038
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetS100B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-S100B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL2720
  • Conjugate
    Unconjugated
  • Gene ID6285
  • Target name
    S100B
  • Target description
    S100 calcium binding protein B
  • Target synonyms
    NEF; protein S100-B; S100; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain; S-100 protein subunit beta; S100-B; S100beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04271
  • Protein Name
    Protein S100-B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of S100B using anti-S100B antibody (M00979-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100B antigen affinity purified monoclonal antibody (Catalog # M00979-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100B at approximately 11 kDa. The expected band size for S100B is at 11 kDa.
Product group Antibodies
References
Boster Bio
Anti-S100B/S100 Beta Rabbit Monoclonal AntibodyM00979-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetS100B
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Product group Antibodies
Assay Genie
S100B AntibodyCAB0676
TargetS100B
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Product group Antibodies
Biomatik
S100 Calcium Binding Protein B (S100B) Monoclonal AntibodyCAU35023
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetS100B
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Product group Antibodies
LifeSpan BioSciences
S100B / S100 Beta Antibody (Preservative Free)LS-C149156
ApplicationsWestern Blot, ELISA
TargetS100B
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA020643ESR1HU at dilution of 1:100
Product group Antibodies
Cusabio
S100B AntibodyCSB-PA020643ESR1HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetS100B
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Product group Antibodies
Biosynth
S100B antibody10R-10377
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Product group Antibodies
RayBiotech
Anti-S100b Antibody130-00023
ApplicationsWestern Blot, ELISA
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Product group Antibodies
Everest Biotech
Goat anti-S100BEB12316
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetS100B
  • SizePrice
Anti-S100B Antibody
Product group Antibodies
Antibodies.com
Anti-S100B AntibodyA28225
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-S100B [1C8]Ab02453-1.1
ApplicationsELISA
TargetS100B
  • SizePrice