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Western blot analysis of lysates from HeLa and Jurkat cells, using SDCG1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa and Jurkat cells, using SDCG1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa and Jurkat cells, using SDCG1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-SDCG1 Antibody

A30462
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNEMF
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-SDCG1 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID9147
  • Target name
    NEMF
  • Target description
    nuclear export mediator factor
  • Target synonyms
    IDDSAPN, NY-CO-1, RQC2, SDCCAG1, ribosome quality control complex subunit NEMF, New-York Colon 1, antigen NY-CO-1, caliban homolog, serologically defined colon cancer antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60524
  • Protein Name
    Ribosome quality control complex subunit NEMF
  • Scientific Description
    Boster Bio Anti-SDCG1 Antibody (Catalog# A30462). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of SDCCAG1/NEMF using anti-SDCCAG1/NEMF antibody (A09039-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDCCAG1/NEMF antigen affinity purified polyclonal antibody (Catalog # A09039-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDCCAG1/NEMF at approximately 140 kDa. The expected band size for SDCCAG1/NEMF is at 123,130-140 kDa.
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