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Immunohistochemical staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.

Anti-SEC23IP-25ul

HPA038403
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEC23IP-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ICC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6Y8
  • Protein Name
    SEC23-interacting protein
  • Scientific Description
    Rabbit Polyclonal Anti-SEC23IP Antibody against Human SEC23 interacting protein. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 292T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC23IP antigen affinity purified polyclonal antibody (Catalog # A09955-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC23IP at approximately 125 kDa. The expected band size for SEC23IP is at 111 kDa.
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