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Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Anti-SEL1L Antibody

HPA024267
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSEL1L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEL1L
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6400
  • Target name
    SEL1L
  • Target description
    SEL1L adaptor subunit of ERAD E3 ubiquitin ligase
  • Target synonyms
    Hrd3; PRO1063; protein sel-1 homolog 1; sel-1 suppressor of lin-12-like 1; SEL1L, ERAD E3 ligase adaptor subunit; SEL1L1; SEL1-LIKE; Suppressor of lin 12 (sel-1), C. elegans, homolog of; suppressor of lin-12-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBV2
  • Protein Name
    Protein sel-1 homolog 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA866208LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of SEL1L using anti-SEL1L antibody (A03343-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEL1L antigen affinity purified polyclonal antibody (Catalog # A03343-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEL1L at approximately 100 kDa. The expected band size for SEL1L is at 89 kDa.
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