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SEL1L Antibody

LS-C668390
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSEL1L
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEL1L Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of Sel-1L expression in HepG2 (A), mouse kidney (B) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of Sel-1L expression in HepG2 (A), mouse kidney (B) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6400
  • Target name
    SEL1L
  • Target description
    SEL1L adaptor subunit of ERAD E3 ubiquitin ligase
  • Target synonyms
    Hrd3; PRO1063; protein sel-1 homolog 1; sel-1 suppressor of lin-12-like 1; SEL1L, ERAD E3 ligase adaptor subunit; SEL1L1; SEL1-LIKE; Suppressor of lin 12 (sel-1), C. elegans, homolog of; suppressor of lin-12-like protein 1
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEL1L using anti-SEL1L antibody (A03343-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEL1L antigen affinity purified polyclonal antibody (Catalog # A03343-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEL1L at approximately 100 kDa. The expected band size for SEL1L is at 89 kDa.
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