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WB analysis of various sample lysates using GTX64717 SEL1L antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64717 SEL1L antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64717 SEL1L antibody. Dilution : 1:3000 Loading : 25microg per lane

SEL1L antibody

GTX64717
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSEL1L
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Overview

  • Supplier
    GeneTex
  • Product Name
    SEL1L antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6400
  • Target name
    SEL1L
  • Target description
    SEL1L adaptor subunit of SYVN1 ubiquitin ligase
  • Target synonyms
    Hrd3, NEDGSAF, NEDHGFA, PRO1063, SEL1-LIKE, SEL1L1, protein sel-1 homolog 1, SEL1L adaptor subunit of ERAD E3 ubiquitin ligase, SEL1L, ERAD E3 ligase adaptor subunit, Suppressor of lin 12 (sel-1), C. elegans, homolog of, sel-1 suppressor of lin-12-like 1, suppressor of lin-12-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBV2
  • Protein Name
    Protein sel-1 homolog 1
  • Scientific Description
    The protein encoded by this gene is part of a protein complex required for the retrotranslocation or dislocation of misfolded proteins from the endoplasmic reticulum lumen to the cytosol, where they are degraded by the proteasome in a ubiquitin-dependent manner. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEL1L using anti-SEL1L antibody (A03343-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEL1L antigen affinity purified polyclonal antibody (Catalog # A03343-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEL1L at approximately 100 kDa. The expected band size for SEL1L is at 89 kDa.
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