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Anti-SEMA4A (N-term) Antibody

102-22446
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSEMA4A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SEMA4A (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB35876
  • Gene ID64218
  • Target name
    SEMA4A
  • Target description
    semaphorin 4A
  • Target synonyms
    CORD10, RP35, SEMAB, SEMB, semaphorin-4A, sema B, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A, semaphorin-B
  • Host
    Rabbit
  • Protein IDQ9H3S1
  • Protein Name
    Semaphorin-4A
  • Scientific Description
    Rabbit Anti-SEMA4A (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEMA4A using anti-SEMA4A antibody (A06779-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse ANA-1 tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA4A antigen affinity purified polyclonal antibody (Catalog # A06779-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA4A at approximately 105 kDa. The expected band size for SEMA4A is at 84,105 kDa.
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