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Western Blot analysis of Jurkat cells using SEMA4A Polyclonal Antibody
Western Blot analysis of Jurkat cells using SEMA4A Polyclonal Antibody
Western Blot analysis of Jurkat cells using SEMA4A Polyclonal Antibody

SEMA4A Antibody

CSB-PA004061
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetSEMA4A
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEMA4A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64218
  • Target name
    SEMA4A
  • Target description
    semaphorin 4A
  • Target synonyms
    CORD10, RP35, SEMAB, SEMB, semaphorin-4A, sema B, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A, semaphorin-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3S1
  • Protein Name
    Semaphorin-4A
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEMA4A using anti-SEMA4A antibody (A06779-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse ANA-1 tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA4A antigen affinity purified polyclonal antibody (Catalog # A06779-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA4A at approximately 105 kDa. The expected band size for SEMA4A is at 84,105 kDa.
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