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Immunohistochemical staining of human retina shows moderate cytoplasmic positivity in nerve fiber layer.
Immunohistochemical staining of human retina shows moderate cytoplasmic positivity in nerve fiber layer.
Immunohistochemical staining of human retina shows moderate cytoplasmic positivity in nerve fiber layer.

Anti-SEMA4F Antibody

HPA064095
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSEMA4F
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEMA4F Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10505
  • Target name
    SEMA4F
  • Target description
    ssemaphorin 4F
  • Target synonyms
    M-SEMA, PRO2353, S4F, SEMAM, SEMAW, m-Sema-M, semaphorin-4F, sema domain immunoglobulin domain transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4F, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F, semaphorin M, semaphorin-W
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95754
  • Protein Name
    Semaphorin-4F
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87 MG whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA4F antigen affinity purified polyclonal antibody (Catalog # A11873-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA4F at approximately 84 kDa. The expected band size for SEMA4F is at 66 kDa.
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