More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SEMA4F / Semaphorin 4F Antibody

SEMA4F / Semaphorin 4F Antibody

LS-C497142
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEMA4F
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEMA4F / Semaphorin 4F Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 66kDa/83kDa, while the observed MW by Western blot was 84kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 66kDa/83kDa, while the observed MW by Western blot was 84kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    6.67 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10505
  • Target name
    SEMA4F
  • Target description
    ssemaphorin 4F
  • Target synonyms
    M-SEMA, PRO2353, S4F, SEMAM, SEMAW, m-Sema-M, semaphorin-4F, sema domain immunoglobulin domain transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4F, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F, semaphorin M, semaphorin-W
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Bioss
SEMA4F Polyclonal AntibodyBS-11478R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Rat
TargetSEMA4F
  • SizePrice
Anti-SEMA4F Antibody
Product group Antibodies
Antibodies.com
Anti-SEMA4F AntibodyA12936
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SEMA4F Antibody144-10432
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSEMA4F
  • SizePrice
WB analysis of A431 cells using GTX46442 SEMA4F antibody at 1:600. Lane 1 (-): A431 cells transfected with control siRNA Lane 2 (+): A431 cells transfected with Sema4F siRNA
Product group Antibodies
GeneTex
SEMA4F antibody, N-termGTX46442
ApplicationsWestern Blot
ReactivityHuman
TargetSEMA4F
  • SizePrice
Immunohistochemical staining of human retina shows moderate cytoplasmic positivity in nerve fiber layer.
Product group Antibodies
Atlas Antibodies
Anti-SEMA4F AntibodyHPA064095
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetSEMA4F
  • SizePrice
Immunofluorescence staining of MCF-7 cells with CSB-PA020992LA01HU at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
SEMA4F AntibodyCSB-PA020992LA01HU
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetSEMA4F
  • SizePrice
Figure 1. Western blot analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87 MG whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA4F antigen affinity purified polyclonal antibody (Catalog # A11873-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA4F at approximately 84 kDa. The expected band size for SEMA4F is at 66 kDa.
Product group Antibodies
Boster Bio
Anti-SEMA4F Antibody Picoband(r)A11873-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSEMA4F
  • SizePrice