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Immunofluorescence staining of MCF-7 cells with CSB-PA020992LA01HU at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of MCF-7 cells with CSB-PA020992LA01HU at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of MCF-7 cells with CSB-PA020992LA01HU at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SEMA4F Antibody

CSB-PA020992LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetSEMA4F
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEMA4F Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10505
  • Target name
    SEMA4F
  • Target description
    ssemaphorin 4F
  • Target synonyms
    M-SEMA, PRO2353, S4F, SEMAM, SEMAW, m-Sema-M, semaphorin-4F, sema domain immunoglobulin domain transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4F, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F, semaphorin M, semaphorin-W
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95754
  • Protein Name
    Semaphorin-4F
  • Scientific Description
    Has growth cone collapse activity against retinal ganglion-cell axons.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87 MG whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA4F antigen affinity purified polyclonal antibody (Catalog # A11873-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA4F at approximately 84 kDa. The expected band size for SEMA4F is at 66 kDa.
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