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anti-SERPINB1 antibody

ARG58622
Arigo Biolaboratories
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSERPINB1
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-SERPINB1 antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1992
  • Target name
    SERPINB1
  • Target description
    serpin family B member 1
  • Target synonyms
    EI; ELANH2; epididymis luminal protein 57; epididymis secretory protein Li 27; HEL57; HEL-S-27; LEI; leukocyte elastase inhibitor; M/NEI; MNEI; peptidase inhibitor 2; PI2; PI-2; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1; serpin peptidase inhibitor, clade B (ovalbumin), member 1
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PI2/SERPINB1 using anti-PI2/SERPINB1 antibody (A04491-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI2/SERPINB1 antigen affinity purified polyclonal antibody (Catalog # A04491-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI2/SERPINB1 at approximately 43 kDa. The expected band size for PI2/SERPINB1 is at 43 kDa.
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