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Anti-SHROOM2 (Center) Antibody

102-22918
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSHROOM2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SHROOM2 (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB37634
  • Gene ID357
  • Target name
    SHROOM2
  • Target description
    shroom family member 2
  • Target synonyms
    apical-like protein; APX homolog of Xenopus; APXL; HSAPXL; protein Shroom2
  • Host
    Rabbit
  • Protein IDQ13796
  • Protein Name
    Protein Shroom2
  • Scientific Description
    Rabbit Anti-SHROOM2 (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SHROOM2 using anti-SHROOM2 antibody (A10015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM2 antigen affinity purified polyclonal antibody (Catalog # A10015-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM2 at approximately 180 kDa. The expected band size for SHROOM2 is at 176 kDa.
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