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ProductsBack/APXL antibody [C3], C-term
Immunohistochemical analysis of paraffin-embedded human brain, cortex, using APXL(GTX100055) antibody(10 μg/ml). Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human brain, cortex, using APXL(GTX100055) antibody(10 μg/ml). Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human brain, cortex, using APXL(GTX100055) antibody(10 μg/ml). Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

APXL antibody [C3], C-term

GTX100055
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetSHROOM2
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Overview

  • Supplier
    GeneTex
  • Product Name
    APXL antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID357
  • Target name
    SHROOM2
  • Target description
    shroom family member 2
  • Target synonyms
    APXL, HSAPXL, protein Shroom2, APX homolog of Xenopus, apical-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13796
  • Protein Name
    Protein Shroom2
  • Scientific Description
    The protein encoded by this gene shares significant similarities with the apical protein from Xenopus laevis which is implicated in amiloride-sensitive sodium channel activity. This gene is a strong candidate gene for ocular albinism type 1 syndrome. [provided by RefSeq]
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Badu-Nkansah KA, Lechler T. Proteomic analysis of desmosomes reveals novel components required for epidermal integrity. Mol Biol Cell. 2020,31(11):1140-1153. doi: 10.1091/mbc.E19-09-0542
    Read this paper
  • Amano M, Hamaguchi T, Shohag MH, et al. Kinase-interacting substrate screening is a novel method to identify kinase substrates. J Cell Biol. 2015,209(6):895-912. doi: 10.1083/jcb.201412008
    Read this paper

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Figure 1. Western blot analysis of SHROOM2 using anti-SHROOM2 antibody (A10015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM2 antigen affinity purified polyclonal antibody (Catalog # A10015-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM2 at approximately 180 kDa. The expected band size for SHROOM2 is at 176 kDa.
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