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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA132880(SHROOM2 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA132880(SHROOM2 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA132880(SHROOM2 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)

SHROOM2 Antibody

CSB-PA132880
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSHROOM2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SHROOM2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID357
  • Target name
    SHROOM2
  • Target description
    shroom family member 2
  • Target synonyms
    APXL, HSAPXL, protein Shroom2, APX homolog of Xenopus, apical-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13796
  • Protein Name
    Protein Shroom2
  • Scientific Description
    The protein encoded by this gene shares significant similarities with the apical protein from Xenopus laevis which is implicated in amiloride-sensitive sodium channel activity. This gene is a strong candidate gene for ocular albinism type 1 syndrome.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SHROOM2 using anti-SHROOM2 antibody (A10015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM2 antigen affinity purified polyclonal antibody (Catalog # A10015-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM2 at approximately 180 kDa. The expected band size for SHROOM2 is at 176 kDa.
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