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Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.

Anti-SHROOM2 Antibody

HPA061435
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSHROOM2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SHROOM2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID357
  • Target name
    SHROOM2
  • Target description
    shroom family member 2
  • Target synonyms
    APXL, HSAPXL, protein Shroom2, APX homolog of Xenopus, apical-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13796
  • Protein Name
    Protein Shroom2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SHROOM2 using anti-SHROOM2 antibody (A10015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM2 antigen affinity purified polyclonal antibody (Catalog # A10015-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM2 at approximately 180 kDa. The expected band size for SHROOM2 is at 176 kDa.
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