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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.

Anti-SKP1 Antibody

HPA058134
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSKP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SKP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6500
  • Target name
    SKP1
  • Target description
    S-phase kinase associated protein 1
  • Target synonyms
    EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A, S-phase kinase-associated protein 1, OCP-2, RNA polymerase II elongation factor-like protein OCP2, SIII, cyclin A/CDK2-associated p19, cyclin-A/CDK2-associated protein p19, organ of Corti protein 2, organ of Corti protein II, p19skp1, transcription elongation factor B polypeptide 1-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63208
  • Protein Name
    S-phase kinase-associated protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SKP1 using anti-SKP1 antibody (A00476-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP1 antigen affinity purified polyclonal antibody (Catalog # A00476-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKP1 at approximately 19KD. The expected band size for SKP1 is at 19KD.
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