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Western Blot analysis of COS7 cells using Skp1 p19 Polyclonal Antibody
Western Blot analysis of COS7 cells using Skp1 p19 Polyclonal Antibody
Western Blot analysis of COS7 cells using Skp1 p19 Polyclonal Antibody

SKP1 Antibody

CSB-PA004097
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetSKP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SKP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6500
  • Target name
    SKP1
  • Target description
    S-phase kinase associated protein 1
  • Target synonyms
    cyclin A/CDK2-associated p19; cyclin-A/CDK2-associated protein p19; EMC19; OCP2; OCP-2; OCP-II; organ of Corti protein 2; organ of Corti protein II; p19A; p19skp1; RNA polymerase II elongation factor-like protein OCP2; SIII; SKP1A; S-phase kinase-associated protein 1; TCEB1L; transcription elongation factor B polypeptide 1-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63208
  • Protein Name
    S-phase kinase-associated protein 1
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SKP1 using anti-SKP1 antibody (A00476-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP1 antigen affinity purified polyclonal antibody (Catalog # A00476-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKP1 at approximately 19KD. The expected band size for SKP1 is at 19KD.
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