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Immunohistochemical staining of human kidney shows strong cytopmasmic positivity with granular pattern in cells in tubules and cells in glomeruli.
Immunohistochemical staining of human kidney shows strong cytopmasmic positivity with granular pattern in cells in tubules and cells in glomeruli.
Immunohistochemical staining of human kidney shows strong cytopmasmic positivity with granular pattern in cells in tubules and cells in glomeruli.

Anti-SLC25A10 Antibody

HPA023048
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSLC25A10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC25A10
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1468
  • Target name
    SLC25A10
  • Target description
    solute carrier family 25 member 10
  • Target synonyms
    DIC; dicarboxylate ion carrier; mitochondrial dicarboxylate carrier; MTDPS19; solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBX3
  • Protein Name
    Mitochondrial dicarboxylate carrier
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antigen affinity purified polyclonal antibody (Catalog # A10727-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitochondrial Dicarboxylate Carrier/SLC25A10 at approximately 29-31 kDa. The expected band size for Mitochondrial Dicarboxylate Carrier/SLC25A10 is at 31 kDa.
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Western blot All lanes: SLC25A10 antibody at 16microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32, 33 kDa Observed band size: 32, 24 kDa
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