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IHC-P analysis of human kidney tissue using GTX51583 SLC25A10 antibody. Dilution : 1:200
IHC-P analysis of human kidney tissue using GTX51583 SLC25A10 antibody. Dilution : 1:200
IHC-P analysis of human kidney tissue using GTX51583 SLC25A10 antibody. Dilution : 1:200

SLC25A10 antibody

GTX51583
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSLC25A10
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLC25A10 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50-400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1468
  • Target name
    SLC25A10
  • Target description
    solute carrier family 25 member 10
  • Target synonyms
    DIC; dicarboxylate ion carrier; mitochondrial dicarboxylate carrier; MTDPS19; solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBX3
  • Protein Name
    Mitochondrial dicarboxylate carrier
  • Scientific Description
    This gene encodes a member of a family of proteins that translocate small metabolites across the mitochondrial membrane. The encoded protein exchanges dicarboxylates, such as malate and succinate, for phosphate, sulfate, and other small molecules, thereby providing substrates for metabolic processes including the Krebs cycle and fatty acid synthesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antigen affinity purified polyclonal antibody (Catalog # A10727-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitochondrial Dicarboxylate Carrier/SLC25A10 at approximately 29-31 kDa. The expected band size for Mitochondrial Dicarboxylate Carrier/SLC25A10 is at 31 kDa.
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