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Western blot All lanes: SLC25A10 antibody at 16microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32, 33 kDa Observed band size: 32, 24 kDa
Western blot All lanes: SLC25A10 antibody at 16microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32, 33 kDa Observed band size: 32, 24 kDa
Western blot All lanes: SLC25A10 antibody at 16microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32, 33 kDa Observed band size: 32, 24 kDa

SLC25A10 Antibody

CSB-PA883371LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC25A10
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC25A10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1468
  • Target name
    SLC25A10
  • Target description
    solute carrier family 25 member 10
  • Target synonyms
    DIC; dicarboxylate ion carrier; mitochondrial dicarboxylate carrier; MTDPS19; solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBX3
  • Protein Name
    Mitochondrial dicarboxylate carrier
  • Scientific Description
    Involved in translocation of malonate, malate and succinate in exchange for phosphate, sulfate, sulfite or thiosulfate across mitochondrial inner membrane.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antigen affinity purified polyclonal antibody (Catalog # A10727-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitochondrial Dicarboxylate Carrier/SLC25A10 at approximately 29-31 kDa. The expected band size for Mitochondrial Dicarboxylate Carrier/SLC25A10 is at 31 kDa.
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