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Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to mitochondria.
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to mitochondria.
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to mitochondria.

Anti-SLC3A1 Antibody

HPA071102
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC3A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC3A1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6519
  • Target name
    SLC3A1
  • Target description
    solute carrier family 3 member 1
  • Target synonyms
    ATR1, CSNU1, D2H, NBAT, RBAT, amino acid transporter heavy chain SLC3A1, B(0,+)-type amino acid transport protein, amino acid transporter 1, b(0,+)-type amino acid transporter-related heavy chain, neutral and basic amino acid transport protein rBAT, solute carrier family 3 (amino acid transporter heavy chain), member 1, solute carrier family 3 (cystine, dibasic and neutral amino acid transporters), member 1, solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07837
  • Protein Name
    Neutral and basic amino acid transport protein rBAT
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SLC3A1 using anti-SLC3A1 antibody (A02654-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse HBZY whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC3A1 antigen affinity purified polyclonal antibody (Catalog # A02654-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC3A1 at approximately 110 kDa. The expected band size for SLC3A1 is at 79 kDa.
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