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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

Anti-SLC8A1 Antibody

HPA070007
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSLC8A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC8A1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6546
  • Target name
    SLC8A1
  • Target description
    solute carrier family 8 member A1
  • Target synonyms
    Na(+)/Ca(2+)-exchange protein 1; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; NCX1; sodium/calcium exchanger 1; solute carrier family 8 (sodium/calcium exchanger), member 1; solute carrier family 8 member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32418
  • Protein Name
    Sodium/calcium exchanger 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCX1/SLC8A1 using anti-NCX1/SLC8A1 antibody (A03876-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCX1/SLC8A1 antigen affinity purified polyclonal antibody (Catalog # A03876-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCX1/SLC8A1 at approximately 120 kDa. The expected band size for NCX1/SLC8A1 is at 120 kDa.
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Lane 1: Human U251 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates probed with NCX1/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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