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Western Blot analysis of 293 cells using NCX1 Polyclonal Antibody
Western Blot analysis of 293 cells using NCX1 Polyclonal Antibody
Western Blot analysis of 293 cells using NCX1 Polyclonal Antibody

SLC8A1 Antibody

CSB-PA004751
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC8A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC8A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6546
  • Target name
    SLC8A1
  • Target description
    solute carrier family 8 member A1
  • Target synonyms
    NCX1, sodium/calcium exchanger 1, Na(+)/Ca(2+)-exchange protein 1, Na+/Ca++ exchanger, Na+/Ca2+ exchanger, solute carrier family 8 (sodium/calcium exchanger), member 1, solute carrier family 8 member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32418
  • Protein Name
    Sodium/calcium exchanger 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCX1/SLC8A1 using anti-NCX1/SLC8A1 antibody (A03876-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCX1/SLC8A1 antigen affinity purified polyclonal antibody (Catalog # A03876-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCX1/SLC8A1 at approximately 120 kDa. The expected band size for NCX1/SLC8A1 is at 120 kDa.
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Lane 1: Human U251 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates probed with NCX1/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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SLC8A1 AntibodyCAB5583
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