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ProductsBack/NCX1 Polyclonal Antibody
Lane 1: Human U251 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates probed with NCX1/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U251 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates probed with NCX1/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U251 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates probed with NCX1/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

NCX1 Polyclonal Antibody

bs-1550R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
TargetSLC8A1
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Overview

  • Supplier
    Bioss
  • Product Name
    NCX1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6546
  • Target name
    SLC8A1
  • Target description
    solute carrier family 8 member A1
  • Target synonyms
    NCX1, sodium/calcium exchanger 1, Na(+)/Ca(2+)-exchange protein 1, Na+/Ca++ exchanger, Na+/Ca2+ exchanger, solute carrier family 8 (sodium/calcium exchanger), member 1, solute carrier family 8 member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of NCX1/SLC8A1 using anti-NCX1/SLC8A1 antibody (A03876-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCX1/SLC8A1 antigen affinity purified polyclonal antibody (Catalog # A03876-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCX1/SLC8A1 at approximately 120 kDa. The expected band size for NCX1/SLC8A1 is at 120 kDa.
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