Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smac/Diablo Rabbit Monoclonal Antibody (Clone RM271) at a 1:1000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smac/Diablo Rabbit Monoclonal Antibody (Clone RM271) at a 1:1000 dilution.
anti-Smac/Diablo (human), Rabbit Monoclonal (RM271)
REV-31-1152-00
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDIABLO
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-Smac/Diablo (human), Rabbit Monoclonal (RM271)
- Delivery Days Customer5
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM271
- Gene ID56616
- Target nameDIABLO
- Target descriptiondiablo IAP-binding mitochondrial protein
- Target synonymsDFNA64; diablo homolog, mitochondrial; diablo-like protein; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase; SMAC
- HostRabbit
- IsotypeIgG
- Scientific DescriptionRecombinant Antibody. This antibody reacts to human Smac (Diablo homolog, mitochondrial). Applications: WB, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis. Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases. This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3. Furthermore, Smac/DIABLO plays an important regulatory role in the sensitization of cancer cells to both immune-and drug-induced apoptosis. It promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway and acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). - Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis. Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases. This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3. Furthermore, Smac/DIABLO plays an important regulatory role in the sensitization of cancer cells to both immune-and drug-induced apoptosis. It promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway and acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203