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ProductsBack/Smac Polyclonal Antibody
Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-10014R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 40kD. Observed band size: 40kD
Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-10014R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 40kD. Observed band size: 40kD
Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-10014R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 40kD. Observed band size: 40kD

Smac Polyclonal Antibody

BS-1298R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDIABLO
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Smac/DIABLO Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID56616
  • Target name
    DIABLO
  • Target description
    diablo IAP-binding mitochondrial protein
  • Target synonyms
    DFNA64; diablo homolog, mitochondrial; diablo-like protein; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase; SMAC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR28
  • Protein Name
    Diablo homolog, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Smac/Diablo using anti-Smac/Diablo antibody (PB9860). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: 22RV1 whole cell lysates, Lane 3: SKOV whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smac/Diablo antigen affinity purified polyclonal antibody (Catalog # PB9860) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smac/Diablo at approximately 25 kDa. The expected band size for Smac/Diablo is at 27 kDa.
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