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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA592293(DIABLO Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA592293(DIABLO Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA592293(DIABLO Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200)

DIABLO Antibody

CSB-PA592293
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDIABLO
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Overview

  • Supplier
    Cusabio
  • Product Name
    DIABLO Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56616
  • Target name
    DIABLO
  • Target description
    diablo IAP-binding mitochondrial protein
  • Target synonyms
    DFNA64; diablo homolog, mitochondrial; diablo-like protein; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase; SMAC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR28
  • Protein Name
    Diablo homolog, mitochondrial
  • Scientific Description
    This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Smac/Diablo using anti-Smac/Diablo antibody (PB9860). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: 22RV1 whole cell lysates, Lane 3: SKOV whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smac/Diablo antigen affinity purified polyclonal antibody (Catalog # PB9860) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smac/Diablo at approximately 25 kDa. The expected band size for Smac/Diablo is at 27 kDa.
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