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Immunohistochemical staining of human ovarian cancer shows strong nuclear positivity in tumor cells.
Immunohistochemical staining of human ovarian cancer shows strong nuclear positivity in tumor cells.
Immunohistochemical staining of human ovarian cancer shows strong nuclear positivity in tumor cells.

Anti-SMARCB1 Antibody

AMAB91919
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMARCB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SMARCB1
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL13973
  • Conjugate
    Unconjugated
  • Gene ID6598
  • Target name
    SMARCB1
  • Target description
    SWI/SNF related BAF chromatin remodeling complex subunit B1
  • Target synonyms
    BAF47, CSS3, INI-1, INI1, MRD15, PPP1R144, RDT, RTPS1, SNF5, SNF5L1, SWNTS1, Sfh1p, Snr1, hSNFS, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, BRG1-associated factor 47, SNF5 homolog, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, SWI/SNF-related matrix-associated protein, hSNF5, integrase interactor 1 protein, malignant rhabdoid tumor suppressor, protein phosphatase 1, regulatory subunit 144, sucrose nonfermenting, yeast, homolog-like 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ12824
  • Protein Name
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of SNF5/SMARCB1 using anti-SNF5/SMARCB1 antibody (A00500-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF5/SMARCB1 antigen affinity purified polyclonal antibody (Catalog # A00500-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF5/SMARCB1 at approximately 44 kDa. The expected band size for SNF5/SMARCB1 is at 44 kDa.
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