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IHC-P analysis of human colon carcinoma tissue using GTX04623 SNF5 antibody [ARC53139]. Antigen retrieval : 10 mM Tris/EDTA buffer pH 9.0 Dilution : 1:100
IHC-P analysis of human colon carcinoma tissue using GTX04623 SNF5 antibody [ARC53139]. Antigen retrieval : 10 mM Tris/EDTA buffer pH 9.0 Dilution : 1:100
IHC-P analysis of human colon carcinoma tissue using GTX04623 SNF5 antibody [ARC53139]. Antigen retrieval : 10 mM Tris/EDTA buffer pH 9.0 Dilution : 1:100

SNF5 antibody [ARC53139]

GTX04623
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSMARCB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SNF5 antibody [ARC53139]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:5000. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ARC53139
  • Conjugate
    Unconjugated
  • Gene ID6598
  • Target name
    SMARCB1
  • Target description
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
  • Target synonyms
    BAF47; BRG1-associated factor 47; CSS3; hSNF5; hSNFS; INI1; integrase interactor 1 protein; malignant rhabdoid tumor suppressor; MRD15; PPP1R144; protein phosphatase 1, regulatory subunit 144; RDT; RTPS1; Sfh1p; SNF5; SNF5 homolog; SNF5L1; Snr1; sucrose nonfermenting, yeast, homolog-like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI/SNF-related matrix-associated protein; SWNTS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12824
  • Protein Name
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
  • Scientific Description
    The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNF5/SMARCB1 using anti-SNF5/SMARCB1 antibody (A00500-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF5/SMARCB1 antigen affinity purified polyclonal antibody (Catalog # A00500-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF5/SMARCB1 at approximately 44 kDa. The expected band size for SNF5/SMARCB1 is at 44 kDa.
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