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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA014989(SMARCB1 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA014989(SMARCB1 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA014989(SMARCB1 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)

SMARCB1 Antibody

CSB-PA014989
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSMARCB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SMARCB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6598
  • Target name
    SMARCB1
  • Target description
    SWI/SNF related BAF chromatin remodeling complex subunit B1
  • Target synonyms
    BAF47, CSS3, INI-1, INI1, MRD15, PPP1R144, RDT, RTPS1, SNF5, SNF5L1, SWNTS1, Sfh1p, Snr1, hSNFS, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, BRG1-associated factor 47, SNF5 homolog, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, SWI/SNF-related matrix-associated protein, hSNF5, integrase interactor 1 protein, malignant rhabdoid tumor suppressor, protein phosphatase 1, regulatory subunit 144, sucrose nonfermenting, yeast, homolog-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12824
  • Protein Name
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
  • Scientific Description
    The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SNF5/SMARCB1 using anti-SNF5/SMARCB1 antibody (A00500-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF5/SMARCB1 antigen affinity purified polyclonal antibody (Catalog # A00500-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF5/SMARCB1 at approximately 44 kDa. The expected band size for SNF5/SMARCB1 is at 44 kDa.
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