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Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.

Anti-SMARCB1 Antibody

HPA019127
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, Chip-exoSeq
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSMARCB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SMARCB1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry, Chip-exoSeq
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6598
  • Target name
    SMARCB1
  • Target description
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
  • Target synonyms
    BAF47; BRG1-associated factor 47; CSS3; hSNF5; hSNFS; INI1; integrase interactor 1 protein; malignant rhabdoid tumor suppressor; MRD15; PPP1R144; protein phosphatase 1, regulatory subunit 144; RDT; RTPS1; Sfh1p; SNF5; SNF5 homolog; SNF5L1; Snr1; sucrose nonfermenting, yeast, homolog-like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI/SNF-related matrix-associated protein; SWNTS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12824
  • Protein Name
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNF5/SMARCB1 using anti-SNF5/SMARCB1 antibody (A00500-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF5/SMARCB1 antigen affinity purified polyclonal antibody (Catalog # A00500-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF5/SMARCB1 at approximately 44 kDa. The expected band size for SNF5/SMARCB1 is at 44 kDa.
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