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Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Anti-SMC3 Antibody

HPA037411
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSMC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SMC3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9126
  • Target name
    SMC3
  • Target description
    structural maintenance of chromosomes 3
  • Target synonyms
    BAM; basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; chondroitin sulfate proteoglycan 6 (bamacan); chromosome-associated polypeptide; CSPG6; HCAP; SMC protein 3; SMC3L1; structural maintenance of chromosomes protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQE7
  • Protein Name
    Structural maintenance of chromosomes protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMC3 using anti-SMC3 antibody (PB9746). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Liver Tissue Lysate at 50ug, Lane 3: Rat Testis Tissue Lysate at 50ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: A549 Whole Cell Lysate at 40ug, Lane 6: MCF-7 Whole Cell Lysate at 40ug, Lane 7: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC3 antigen affinity purified polyclonal antibody (Catalog # PB9746) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC3 at approximately 140 kDa. The expected band size for SMC3 is at 140 kDa.
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