![Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SMC3 antibody [N1N2], N-term (GTX109827) at 1:300 dilution. Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SMC3 antibody [N1N2], N-term (GTX109827) at 1:300 dilution.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109827/GTX109827_40030_IHC_Z_22111423_427.webp)
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SMC3 antibody [N1N2], N-term (GTX109827) at 1:300 dilution.
SMC3 antibody [N1N2], N-term
GTX109827
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Zebra Fish
TargetSMC3
Overview
- SupplierGeneTex
- Product NameSMC3 antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.95 mg/ml
- ConjugateUnconjugated
- Gene ID9126
- Target nameSMC3
- Target descriptionstructural maintenance of chromosomes 3
- Target synonymsBAM, BMH, CDLS3, CSPG6, HCAP, SMC3L1, structural maintenance of chromosomes protein 3, SMC protein 3, basement membrane-associated chondroitin proteoglycan, chondroitin sulfate proteoglycan 6 (bamacan), chromosome-associated polypeptide
- HostRabbit
- IsotypeIgG
- Protein IDQ9UQE7
- Protein NameStructural maintenance of chromosomes protein 3
- Scientific DescriptionThis gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq]
- ReactivityDrosophila, Human, Mouse, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161

![Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [N1N2], N-term (GTX109827) or SMC3 antibody [C2C3], C-term (GTX110402). Western blot analysis was performed using SMC3 antibody [N1N2], N-term (GTX109827). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [N1N2], N-term (GTX109827) or SMC3 antibody [C2C3], C-term (GTX110402). Western blot analysis was performed using SMC3 antibody [N1N2], N-term (GTX109827). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109827/GTX109827_40030_20141119_IP_w_23060500_262.webp)

![Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [N1N2], N-term (GTX109827). Western blot analysis was performed using SMC3 antibody [N1N2], N-term (GTX109827) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [N1N2], N-term (GTX109827). Western blot analysis was performed using SMC3 antibody [N1N2], N-term (GTX109827) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109827/GTX109827_40030_IP_w_23060500_466.webp)

![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC3 antibody [N1N2], N-term (GTX109827) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC3 antibody [N1N2], N-term (GTX109827) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109827/GTX109827_40030_20230630_WB_Drosophila_23062718_592.webp)





