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ProductsBack/Structural Maintenance Of Chromosomes Protein 3 (SMC3) Polyclonal Antibody

Structural Maintenance Of Chromosomes Protein 3 (SMC3) Polyclonal Antibody

CAU26460
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSMC3
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Overview

  • Supplier
    Biomatik
  • Product Name
    Structural Maintenance Of Chromosomes Protein 3 (SMC3) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9126
  • Target name
    SMC3
  • Target description
    structural maintenance of chromosomes 3
  • Target synonyms
    BAM, BMH, CDLS3, CSPG6, HCAP, SMC3L1, structural maintenance of chromosomes protein 3, SMC protein 3, basement membrane-associated chondroitin proteoglycan, chondroitin sulfate proteoglycan 6 (bamacan), chromosome-associated polypeptide
  • Host
    Rabbit
  • Protein IDQ9UQE7
  • Protein Name
    Structural maintenance of chromosomes protein 3
  • Scientific Description
    The Structural Maintenance Of Chromosomes Protein 3 (SMC3) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMC3 using anti-SMC3 antibody (PB9746). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Liver Tissue Lysate at 50ug, Lane 3: Rat Testis Tissue Lysate at 50ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: A549 Whole Cell Lysate at 40ug, Lane 6: MCF-7 Whole Cell Lysate at 40ug, Lane 7: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC3 antigen affinity purified polyclonal antibody (Catalog # PB9746) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC3 at approximately 140 kDa. The expected band size for SMC3 is at 140 kDa.
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