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Immunohistochemical staining of human testis shows weak nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows weak nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows weak nuclear positivity in cells in seminiferous ducts.

Anti-SMC6 Antibody

HPA042733
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMC6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SMC6 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79677
  • Target name
    SMC6
  • Target description
    structural maintenance of chromosomes 6
  • Target synonyms
    SMC-6, SMC6L1, hSMC6, structural maintenance of chromosomes protein 6, SMC protein 6, SMC6 structural maintenance of chromosomes 6-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96SB8
  • Protein Name
    Structural maintenance of chromosomes protein 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human COLO-320 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC6L1 antigen affinity purified polyclonal antibody (Catalog # A01554-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC6L1 at approximately 126KD. The expected band size for SMC6L1 is at 126KD.
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