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SMC6 antibody

70R-20401
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    SMC6 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA571155(SMC6 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human COLO-320 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC6L1 antigen affinity purified polyclonal antibody (Catalog # A01554-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC6L1 at approximately 126KD. The expected band size for SMC6L1 is at 126KD.
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Anti-SMC6L1 Antibody Picoband(r)A01554-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
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TargetSMC6
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Immunohistochemical staining of human testis shows weak nuclear positivity in cells in seminiferous ducts.
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Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SMC6 Polyclonal Antibody, Unconjugated (bs-7730R) at 1:200 overnight at 4°C, DAB staining.
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Bioss
SMC6 Polyclonal AntibodyBS-7730R
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SMC6L1 antibody [N3C2], Internal detects SMC6 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMC6 protein stained by SMC6L1 antibody [N3C2], Internal (GTX116832) diluted at 1:500. Blue: Hoechst 33342 staining.
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GeneTex
SMC6L1 antibody [N3C2], InternalGTX116832
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetSMC6
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