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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA571155(SMC6 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA571155(SMC6 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA571155(SMC6 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

SMC6 Antibody

CSB-PA571155
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMC6
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Overview

  • Supplier
    Cusabio
  • Product Name
    SMC6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79677
  • Target name
    SMC6
  • Target description
    structural maintenance of chromosomes 6
  • Target synonyms
    SMC-6, SMC6L1, hSMC6, structural maintenance of chromosomes protein 6, SMC protein 6, SMC6 structural maintenance of chromosomes 6-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96SB8
  • Protein Name
    Structural maintenance of chromosomes protein 6
  • Scientific Description
    Structural maintenance of chromosomes protein 6 is a protein that in humans is encoded by the SMC6 gene. Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human COLO-320 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC6L1 antigen affinity purified polyclonal antibody (Catalog # A01554-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC6L1 at approximately 126KD. The expected band size for SMC6L1 is at 126KD.
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TargetSMC6
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References
GeneTex
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TargetSMC6
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Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SMC6 Polyclonal Antibody, Unconjugated (bs-7730R) at 1:200 overnight at 4°C, DAB staining.
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