Figure 1. Western blot analysis of SMURF1 using anti-SMURF1 antibody (PB9892). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF1 antigen affinity purified polyclonal antibody (Catalog # PB9892) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF1 at approximately 86 kDa. The expected band size for SMURF1 is at 86 kDa.
Figure 1. Western blot analysis of SMURF1 using anti-SMURF1 antibody (PB9892). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF1 antigen affinity purified polyclonal antibody (Catalog # PB9892) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF1 at approximately 86 kDa. The expected band size for SMURF1 is at 86 kDa.
Anti-SMURF1 Antibody Picoband(r)
PB9892-DYLIGHT488
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Rat
TargetSMURF1
Overview
- SupplierBoster Bio
- Product NameAnti-SMURF1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 488
- Gene ID57154
- Target nameSMURF1
- Target descriptionSMAD specific E3 ubiquitin protein ligase 1
- Target synonymsE3 ubiquitin ligase SMURF1; E3 ubiquitin-protein ligase SMURF1; HECT-type E3 ubiquitin transferase SMURF1; hSMURF1; Smad ubiquitination regulatory factor 1; Smad-specific E3 ubiquitin ligase 1
- HostRabbit
- IsotypeIgG
- Protein IDQ9HCE7
- Protein NameE3 ubiquitin-protein ligase SMURF1
- Scientific DescriptionBoster Bio Anti-SMURF1 Antibody Picoband® catalog # PB9892. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203