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Anti-SNAI1 Antibody

144-05544
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSNAI1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SNAI1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6615
  • Target name
    SNAI1
  • Target description
    snail family transcriptional repressor 1
  • Target synonyms
    dJ710H13.1; protein sna; protein snail homolog 1; SLUGH2; SNA; SNAH; SNAIL; snail 1 homolog; snail 1 zinc finger protein; snail family zinc finger 1; snail homolog 1; SNAIL1; zinc finger protein SNAI1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95863
  • Protein Name
    Zinc finger protein SNAI1
  • Scientific Description
    SNAI1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Snail1/2/3 Polyclonal Antibody(bs-1371R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss Antibodies
SNAI1 Polyclonal Antibodybs-1371R
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Figure 1. Western blot analysis of SNAIL/SNAI1 using anti-SNAIL/SNAI1 antibody (A00716-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Raji whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAIL/SNAI1 antigen affinity purified polyclonal antibody (Catalog # A00716-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAIL/SNAI1 at approximately 29 kDa. The expected band size for SNAIL/SNAI1 is at 29 kDa.
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Anti-SNAIL/SNAI1 Antibody Picoband(r)A00716-2-CARRIER-FREE
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