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Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Anti-SNX9 Antibody

HPA057203
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSNX9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNX9 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51429
  • Target name
    SNX9
  • Target description
    sorting nexin 9
  • Target synonyms
    SDP1, SH3PX1, SH3PXD3A, WISP, sorting nexin-9, SH3 and PX domain-containing protein 1, SH3 and PX domain-containing protein 3A, Wiskott-Aldrich syndrome protein (WASP) interactor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5X1
  • Protein Name
    Sorting nexin-9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SH3PX1/SNX9 using anti-SH3PX1/SNX9 antibody (A03796-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3PX1/SNX9 antigen affinity purified polyclonal antibody (Catalog # A03796-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3PX1/SNX9 at approximately 78 kDa. The expected band size for SH3PX1/SNX9 is at 67 kDa.
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