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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.

Anti-SOCS1 Antibody

HPA074108
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSOCS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SOCS1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8651
  • Target name
    SOCS1
  • Target description
    suppressor of cytokine signaling 1
  • Target synonyms
    AISIMD, CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP-3, TIP3, suppressor of cytokine signaling 1, JAK binding protein, STAT induced SH3 protein 1, STAT-induced STAT inhibitor 1, Tec-interacting protein 3, cytokine-inducible SH2 protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15524
  • Protein Name
    Suppressor of cytokine signaling 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SOCS1 using anti-SOCS1 antibody (A00448). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS1 antigen affinity purified polyclonal antibody (Catalog # A00448) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS1 at approximately 27 kDa. The expected band size for SOCS1 is at 24 kDa.
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