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Western Blot analysis of KB cells using SOCS-1 Polyclonal Antibody
Western Blot analysis of KB cells using SOCS-1 Polyclonal Antibody
Western Blot analysis of KB cells using SOCS-1 Polyclonal Antibody

SOCS1 Antibody

CSB-PA004129
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSOCS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SOCS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8651
  • Target name
    SOCS1
  • Target description
    suppressor of cytokine signaling 1
  • Target synonyms
    AISIMD, CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP-3, TIP3, suppressor of cytokine signaling 1, JAK binding protein, STAT induced SH3 protein 1, STAT-induced STAT inhibitor 1, Tec-interacting protein 3, cytokine-inducible SH2 protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15524
  • Protein Name
    Suppressor of cytokine signaling 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SOCS1 using anti-SOCS1 antibody (A00448). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS1 antigen affinity purified polyclonal antibody (Catalog # A00448) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS1 at approximately 27 kDa. The expected band size for SOCS1 is at 24 kDa.
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